Flux balance analysis-based metabolic modeling of microbial secondary metabolism: Current status and outlook
Fig 2
Quantitative assessment of existing FBA-based modeling techniques for predicting secondary metabolite production.
(A) Comparison of predicted and observed growth rates and ACT production fluxes at different observed growth rates in Alam and colleagues [87]. (B) Comparison of 4 different objective functions used in FBA to predict both primary metabolism and clavulanic acid production flux [89]. Correlation scores are computed for predicted and observed fluxes. P-lim: limited phosphorus content. (C) Comparison of predicted and observed production fluxes of ACT and RED in Kim and colleagues [97]. ACT, actinorhodin; FBA, flux balance analysis.