A biologically inspired repair mechanism for neuronal reconstructions with a focus on human dendrites
Fig 6
Growth algorithm extends incomplete human CA1 pyramidal cell morphologies.
A, Confocal microscope image of the human hippocampal CA1 region (DG: dentate gyrus; SLM: stratum lacunosum moleculare; SR: stratum radiatum; SP: stratum pyramidale; SO: stratum oriens) with stained pyramidal cells and ROI (region of interest). B, Morphology reconstructions. C, ROI enlarged from A. D, ROI with overlays of originally reconstructed pyramidal cell morphologies by Ref. [26], which are incomplete due to experimental limitations (see text). Scale bar = 460 μm in A, B. E, ROI showing morphologies from D that have been artificially extended in the apical and basal arbour, showing plausible completion of incomplete dendrites based on known layer-specific target growth regions. Each individual neuron has been given a different colour to distinguish the morphologies.