A biologically inspired repair mechanism for neuronal reconstructions with a focus on human dendrites
Fig 5
The repair algorithm successfully recovered artificially removed dendrites from mouse CA1 pyramidal cells and restored their Sholl profiles.
A, Three example repairs of apical and basal dendrites of mouse CA1 pyramidal neurons [Reconstructions from 26]. For each repair, the left morphology is the reconstructed reference with cut branches in magenta and the right morphology is the repaired tree with regrown branches in green. The area enclosed by the dashed black line represents the 3D volume into which the artificial dendrites grew, corresponding to the convex hull of the severed dendrites (see Methods). The graphs below each repair show the distributions of Sholl intersections for the Cut, Repaired and Reference morphologies. B, Each graph shows the value of the repaired morphology (green dots) plotted against the value of the original morphology in black on the identity line. For comparison, the data points in magenta show the values for the cut morphologies. Top, left to right Total number of branch points, Total dendritic length, Average dendritic length per segment in the apical dendrite (one segment is measured from one branch point to the next). Bottom, left to right Average dendritic length per segment in the basal dendrite, Average diameter per segment in the apical dendrite, Average diameter per segment in the basal dendrite. The root mean square errors (RMSE) show the deviation from the reference values as a percentage of the reference value.