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Gentle and fast all-atom model refinement to cryo-EM densities via a maximum likelihood approach

Fig 4

Refinement into noise-free data with adaptive force scaling.

a Aligned (left) and unaligned (right) starting conformations (black sticks) of a helix subject to refinement simulation into a synthetically generated cryo-EM density (gray mesh). b RMSD per residue of the final refined models starting from the aligned conformation compared to the ground truth model underlying the synthetic density map. Each replicate (n=7) is colored by the similarity measure used, inner-product (purple), cross-correlation (ochre), relative-entropy swapped (dark blue), and relative-entropy (green). c RMSD per residue of the final refined models starting from the unaligned conformation compared to the ground truth model underlying the synthetic density map. Each replicate (n=7) is colored by the similarity measure used, inner-product (purple), cross-correlation (ochre), relative-entropy swapped (dark blue), and relative-entropy (green).

Fig 4

doi: https://doi.org/10.1371/journal.pcbi.1011255.g004