MetaNovo: An open-source pipeline for probabilistic peptide discovery in complex metaproteomic datasets
Fig 5
Percentages of misassigned peptides for all three 9MM runs.
A. MetaNovo originally yielded a very high percentage of misassigned peptides at species level UniPept pept2lca analysis. B. Taxonomic breakdown of misassigned peptides C. Re-analysis after inclusion of plausible taxa yielded a species-level misassignment rate of only 1.04%, with 0% error for all approaches at genus and family level using the 0.5% taxon-specific peptide stringency cutoff. D. 9 Acidobacteria bacterium peptides making up the final misassignment percentage of MetaNovo.