Simulation-based Reconstructed Diffusion unveils the effect of aging on protein diffusion in Escherichia coli
Fig 5
Cell pole analysis of diffusion.
(A) Comparison of the ratios of the diffusion coefficients at the poles and center of the cell for data analyzed by SbRD and SMdM. The black dotted line shows the case when the diffusion at the poles and center is equal. For simulated data (orange) the ratio obtained with SbRD is equal to 1 for each protein. For microscopy data (blue) we find a positive correlation between the ratio obtained by SMdM and SbRD. (B) Ratios of diffusion at the poles and the cell center obtained by SMdM and SbRD. (C) Ratios of diffusion at the poles and cell center by SbRD for control cells, and cells treated with 250 μg/ml of erythromycin or 500 μg/ml rifampicin. (D) Diffusion coefficients at the cell center (blue) and poles (orange) for control, erythromycin- and rifampicin-treated cells. (E) Distribution of differences in diffusion coefficients between the newly formed cell pole and the old cell pole. The orange line represents the average of the distribution, the black dashed line is the zero. All curves are obtained via kernel density estimation. Statistical significance is indicated with asterisks.