Comparative Clustering (CompaCt) of eukaryote complexomes identifies novel interactions and sheds light on protein complex evolution
Fig 6
(A) Overview of CompaCt output cluster containing proteins of the p24 family in Y. lipolytica, H. sapiens and P. falciparum. Clustered proteins with a fraction clustered score of over 0.5 or best hit homologs of those are displayed. Proteins in columns are orthologs or best hit homologs with two or more proteins in the same block, reflecting likely gene duplications. (B) Phylogenetic tree of all representatives of the p24 protein family in the three aforementioned species. Species-specific branches have been colored. Members of the protein family that were not detected in the data used in this project are shown in gray. Sequences were aligned with ClustalOmega [39], and the phylogenetic tree was reconstructed with PhyML [40]. The dynamic evolution of the protein family whose members are part of one complex are well captured by the CompaCt approach.