Data-driven segmentation of cortical calcium dynamics
Fig 10
Validation of domain maps by functional activity during locomotion.
(A) Behavioral video was used to extract locomotion/treadmill (red) and whisking (orange) time course. Boxed regions show analysis via optic flow previously described [41] (B) Correlation domain maps shown with either the re-addition of the original mean (left) and the filtered mean (right). Scatter plot showing the fraction of time walking or whisking (left) and correlation of the global mean to locomotion (right). (C) Comparison of the original mean (top; blue) and filtered mean (black) to the treadmill (red) and whisking (orange) time courses, and individual domain time courses with either the filtered (black) or original (blue) mean re-added that correlated to locomotion or were anticorrelated/less correlated. (D) Average domain map with global mean (left) and filtered mean (right) readded, functionally discrete regions highlighted. (E) Cluster analysis on the full movie (left) or just the times when locomotion was occurring (right). The co-clustering of the frontal motor region with barrel fields occurred in 6/7 mice.