Data-driven segmentation of cortical calcium dynamics
Fig 9
Domain maps are created from ICA components and are unique to each recording, but highly similar among individual animals.
(A) Domain size (circle size) and minor axis (green-purple colorbar) plotted at each position across all hemispheres independently (left). Highest eccentric domains (top quartile) plotted showing the location and direction of its major axis (right; n hemispheres = 14, 7 mice) (B) The Allen Brain atlas map [36] is additionally used for anatomical reference. (C) The final manually assigned region, with associated labels. (D) Domain minor axis and eccentricity by region (grey horizontal line denotes top quantile used in A). 7 mice). (E) Example overlay of one domain map on another from the same animal. Individual domain or region overlap is calculated using the Jaccard index (intersect / union). Population analysis of the Jaccard index for domain (F) and region (G) overlap comparisons. Maps are generated from a different recording on the same animal, a littermate, a non-littermate, or a randomly generated voronoi map. Significance is calculated using a two-way ANOVA, followed by post-hoc t-test analysis with Holm-Sidak correction. Retrosplenial: R; V1: Visual, Higher order visual: V+; Auditory: A; Somatosensory Secondary: Ss; Somatosensory Core: Sc; Somatosensory Barrel: Sb; Somatosensory other: S; Motor medial: Mm; Motor lateral: Ml.