Data-driven segmentation of cortical calcium dynamics
Fig 7
Domain maps represent the features of the ICs.
(A) Schematic of domain map creation. A maximum projection is taken through each blurred neural component to form a domain map. Mean time courses are extracted from rebuilt filtered movies within a defined domain. (B) Left scatter plot compares the number of neuronal ICs and the resulting number of domains and the dashed line is the identity line. The circled dot indicates the examples used in this figure. Right bar plot shows the fraction of ICs that have major contributions to the number of domains. (C) Examples of ICs that contributed two domains, one domain or had no contribution. The location of the maxima (black circle) of each IC was found and a point correlation map of the rebuilt full resolution filtered movie was produced (green and pink maps). Pixel-wise scatter plot (right) shows the relationship between the spatial IC value and the correlation map value. Dotted line is the threshold value described in Fig 4. Red line indicates the median correlation value of the correlation map that resides in the thresholded IC. (D) IC-Domain correlation with domain map created with filtered mean plotted with respect to the size of each domain. Dark blue corresponds to domains where each IC made more than one domain. Bright blue corresponds to those that made only one. (E) Median correlation value based on point-correlation analysis within each domain (top). Difference in correlation between the center domain with all its adjacent neighbors (gray scale value corresponds to the mean of the median difference value found in each immediately adjacent domain). (F) Example time courses of domain neighborhood. Left domain map identifies the location of each neighborhood with each corresponding time series. Example ICs and point correlation data shown in S10 Fig.