MitoTNT: Mitochondrial Temporal Network Tracking for 4D live-cell fluorescence microscopy data
Fig 5
Mitochondrial network remodeling analysis.
a, Representative snapshots of a tracked fusion event (left), and fission event (right). b, Node diffusivity values are significantly lower for randomly selected nodes (green) as compared to nodes undergoing fusion (cyan) and fission (magenta). Student’s t-test are applied, and p-values are 6.565e-25 and 1.237e-24 for random vs. fusion nodes and random vs. fission nodes, respectively. c, Representative tracking of mitochondrial fragments in hiPSCs over three timepoints where each fragment is uniquely colored. d, Analysis of fission/fusion preferences with respect to fragment size for control (blue) and nocodazole-treated condition (green). Normalized network size difference measures the differences in # nodes for the fragments undergoing remodeling events, normalized by # nodes of the larger fragment. Mann-Whitney test is used to test whether the underlying distribution is the same, with p-values = 1.2e-32.