Metheor: Ultrafast DNA methylation heterogeneity calculation from bisulfite read alignments
Fig 4
Association between methylation entropy and cancer stemness.
(A) Genes were ranked by the Pearson’s correlation between their expression and average methylation entropy levels across promoters. Red dots represent 3,680 genes having statistically significant correlations (Benjamini-Hochberg adjusted p-value < 0.05), and the results of functional enrichment analysis using those genes are shown in (B). (C) The distribution of promoter methylation entropy levels in primary and metastatic cancer cell lines. (D) The association between promoter methylation entropy levels and two genes representative of Wnt signaling pathway (WNT7A and CTNND2). (E) The association between promoter methylation entropy levels and the activity of Wnt signaling pathway. *two-tailed independent t-test p < 0.05; In D-E, Pearson’s correlation coefficients and associated p-values are shown. In D, p-values were adjusted using Benjamini-Hochberg procedure.