Funneling modulatory peptide design with generative models: Discovery and characterization of disruptors of calcineurin protein-protein interactions
Fig 5
FP competition assay of selected peptides for the binding of Cn to PVIVIT peptide.
Variable concentrations of each selected peptide were incubated with Cn bound to the FITC-labeled PVIVIT peptide. Polarization levels were read and normalized values were fitted to a single site model. The curve shows the bound fraction of Cn to PVIVIT vs. the logarithmic concentration of the peptides. Red—natural peptides, purple—designed peptides, black—PVIVIT.