chiLife: An open-source Python package for in silico spin labeling and integrative protein modeling
Fig 3
Illustration of how changes in backbone do not necessarily cause changes in distance distributions.
Top: Comparison of apo (blue, PDBID 1OMP) and holo (red, PDBID 1ANF) MBP structures and the locations of the model R1 spin labels (sticks) for the site pairs E38R1 S352R1 (A) and E45R1 S211R1 (B). Bottom: Comparison of apo and holo distance distributions for the two site pairs that both show significant changes in Cβ–Cβ backbone distance, indicated by small triangles at the base of the plots. The E38R1 S352R1 site pair on the left (C) shows a clear difference in the predicted distributions while the E45R1 S211R1 site pair on the right (D) shows very little change.