Close-kin mark-recapture methods to estimate demographic parameters of mosquitoes
Fig 5
Sampling schemes to estimate NA and μA for Ae. aegypti.
Violin plots depict estimates of NA and μA for sampling scenarios described in §3.1. The simulated population consists of 3,000 adult Ae. aegypti with bionomic parameters listed in Table 1. Boxes depict median and interquartile ranges of 100 simulation-and-analysis replicates for each scenario, thin lines represent 5% and 95% quantiles, points represent outliers, and kernel density plots are superimposed. The default sampling scheme consists of 1,000 individuals sampled as ca. 11–12 individuals per day over three months. In panels (A-B), sampled larval, adult female and adult male life stage proportions are varied in 25% increments and limited to scenarios where the number of sampled adult females exceeds the number of sampled adult males (e.g., “75F25M” represents a sample consisting of 75% adult females and 25% adult males, and “50F25M25L” represents a sample consisting of 50% adult females, 25% adult males, and 25% larvae). The case of 100% sampled pupae is also included. In panels (C-D), all sampled individuals are adult females, and four sampling frequencies are considered—daily, biweekly, weekly and fortnightly. In panels (E-F), biweekly sampling is adopted, and sampling durations of 1–4 months are explored. In panels (G-H), a sampling duration of three months is adopted, and total sample sizes of 500, 1,000, 1,500 and 2,000 adult females are explored.