Experimental validation of computerised models of clustering of platelet glycoprotein receptors that signal via tandem SH2 domain proteins
Fig 3
The effect of monovalent, divalent and tetravalent Nb2 on platelet aggregation.
(A) Representative traces of platelets incubated with (i) Nb2-2 (ii) Nb2, 5 min before collagen (3 μg/mL) (iii) Nb2-2, 5 min before collagen (3 μg/mL); (iv) concentration-response relationship of inhibition of aggregation (Agg) to collagen by Nb2 and Nb2-2, results shown as mean + s.d. (B) (i) Representative traces of platelets stimulated by Nb2-4 (ii) concentration response curves with results shown as mean ± s.d. aggregation (%) after 5 min. (iii) Representative traces of platelets incubated with vehicle (0.1% DMSO), PP2 (20 μM), PRT-060318 (10 μM) or Nb2 (100 nM) for 5 min before stimulation with Nb2-4 (16 nM) (iv) histogram showing mean ± s.d. aggregation (%) after 5 min; all five treatment groups were compared using one way ANOVA analysis, followed by Tukey test: **(P < 0.01); n = 3.