Experimental validation of computerised models of clustering of platelet glycoprotein receptors that signal via tandem SH2 domain proteins
Fig 2
Interaction of monovalent, divalent and tetravalent ligands with a monomeric receptor.
(A) The concentration-occupancy relationship at equilibrium of a monovalent ligand (L) and a monomeric receptor (R). The ligand is maintained at a constant concentration (red). The following parameters have been used: KD = 1 μM, k1 = 1 μM-1s-1, k-1 = 1 s-1. (B) The concentration-occupancy relationship at equilibrium for a soluble divalent ligand [L2] and monomeric receptor, varying the second association rate constant (k2): the following values have been used: k1 = 1 μM-1s-1, k-1 and k-2 = 1 s-1 (i) k2 = 0.1 μM-1s-1 (ii) k2 = 1 μM-1s-1 (iii) k2 = 10 μM-1s-1. L2R and L2RR reflects a divalent ligand bound to 1 and 2 receptors, respectively. (C) The concentration-occupancy relationship at equilibrium for a soluble tetravalent ligand (L4) and monomeric receptor varying the concentration of (i) ligand and (ii) receptor; the concentration of L4 in (ii) was 1 μM. The other parameters are: kn = 1 μM-1s-1 and k-n = 1 s-1. The receptor concentration in (A, B and C) is 1 μM. The graphs were generated with the MATLAB code: https://github.com/zeemaqsood/TAPAS_ESR9_Modelling_Project. The selected parameters are for illustrative purposes.