Variant calling enhances the identification of cancer cells in single-cell RNA sequencing data
Fig 2
Heatmap indicating alteration status for 736 cells for the top 25 most frequent oncogenic, predicted oncogenic, and likely oncogenic alterations in the CRC dataset.
Alterations are annotated using OncoKB. Absence of an alteration is noted when a cell has a read depth of at least 5 for all bases corresponding to the residue. For residues without an oncogenic alteration and with read depths less than 5 for all corresponding bases, the presence or absence of an alteration is not characterized (labeled as “Insufficient coverage”). Common recurrent driver mutations are shown in bold.