RNA velocity unraveled
Fig 5
Distortions in data and instabilities in the inferred γ/β values introduced by the imputation procedure on the forebrain data from [1].
Column 1: Raw data (points: spliced and unspliced counts with added jitter; color: cell type, as in Fig 1; line: best fit line u = γs/β + q, estimated from the entire dataset). Columns 2–4: Normalized and imputed data under various values of k (points: spliced and unspliced counts; color: cell type, as in Fig 1; line: best linear fit u = γs/β + q, estimated from extreme quantiles). Column 5: Inferred values of γ/β (red, left axis) and inferred fraction of upregulated cells, defined as ∑iui − (γsi/β + q)>0 (blue, right axis).