Development of a scoring function for comparing simulated and experimental tumor spheroids
Fig 6
Deviation score comparison for four simulated spheroid phenotypes.
a) Shown are the deviation scores for five replicates of each phenotype on the upper triangle, and the average deviation score over all replicates of each phenotype on the lower triangle. A top-down view of the spheroid point cloud for each replicate is shown next to the respective row/column. Blue cells are classified as non-gaslike, and red cells are classified as gaslike. For better comparison, an enlarged version of each spheroid was placed at the bottom of the figure. We observe the highest deviation between the “disordered” phenotype and the others, with the maximum deviation between the “spherical” and the “disordered” phenotypes. The “spherical”, “spherical with far gaslikes” and “deformed” phenotypes, which are more similar from a visual perspective, show a smaller deviation score using our analysis, but are nonetheless distinguishable. b) Box plots of the deviation score values between the “spherical” phenotype and each other phenotype. The values used here correspond to those used for the lowest row of subfigure a). We observe that the deviation scores for the “spherical” phenotype compared with the other phenotypes consistently lie above the maximum deviation score of the “spherical” phenotype compared with itself. Significance was determined using Welch’s t-test.