Model-based translation of DNA damage signaling dynamics across cell types
Fig 4
Reduced p53-MDM2 feedback promotes p53 and MDM2 expression.
(A) Model simulations upon disruption of the MDM2-p53 feedback by weakening the feedback strength of MDM2 on p53 degradation to 50% (dashed green) or 20% (dashed yellow) of the original strength; simulations without disruption are shown as solid purple lines. (B-C) p53-GFP (B) and MDM2-GFP (C) expression in HepG2 cells after exposure to increasing concentrations of cisplatin (purple) and to cisplatin in combination with 5 (green) or 10 (yellow) μM Nutlin, both administered at t = 0 hours.