Orchestrated neuronal migration and cortical folding: A computational and experimental study
Fig 2
Experimental data used for calibration of the computational model.
Top: time alignment between experiments and simulations. Ferrets were in utero electroporated between E31 and E37, and brain sections were prepared and imaged between E39 and P16; these dates correspond to simulation times between 0 and 27 days (0 d to 27 d). Images of ferret brains are reproduced from [34]. Bottom: regions of interest (ROI) taken at consistent locations in N = 8 typically developing ferret brains, grouped based on their imaging timepoint (E39–40, P5–6, and P16). For each timepoint, we show neuron cohorts born at different embryonic times (E31, E33–34, and E36–37) labeled with EGFP (bright green). As expected, younger neurons occupy the outermost of the cortex’s six laminae. For consistency, the data marker used in the results is shown for each of the eight samples; color indicates the cohort (cohort 1 is blue, cohort 2 is purple, and cohort 3 is red) while color saturation indicates the imaging timepoint (with lighter and darker colors representing earlier and later imaging, respectively). Scale bar, 500 μm. For detailed methods on the experimental approach, please refer to Section 4.2.