CellDynaMo–stochastic reaction-diffusion-dynamics model: Application to search-and-capture process of mitotic spindle assembly
Fig 6
Describing chromosome arms and flexible corona surface in Stochastic Reaction-Diffusion-Dynamics Model.
A) Probability to find each type of attachment for a single chromosome (CH) with CH arms. Blue and red bars represent statistics for kinetochore pairs (KTs) without and with CH arms, respectively. The statistics are based on n = 20 simulation runs for each case. B) Snapshot after 30 min simulation of biological time shows a representative example of merotelic attachment. C) Changes in KT-KT orientation angle (solid lines; left y-axis) and distance between KT-pair and equatorial plate (dash-dotted lines, right y-axis) over time for the most representative simulation run for the case study with CH arms present. D) Number of MTs vs. time profiles for the same simulation run as in panel C showing proper amphitelic attachment. Abbreviations used: L1 and L2 denote the numbers of MTs from the left CS attached to the first KT and second KT, respectively; R1 and R2 are the numbers of MTs from the right CS attached to the first KT and second KTs, respectively. E) Probability to find each type of attachment for a rigid corona surface (blue) and for a flexible corona surface (red). The statistics are based on n = 20 simulation runs for each case study. F) KT-MT interface and corona flexing for a case of amphitelic attachment. G) KT-MT interface and corona flexing for a case of merotelic attachment.