CellDynaMo–stochastic reaction-diffusion-dynamics model: Application to search-and-capture process of mitotic spindle assembly
Fig 2
Kinetochore-microtubule attachments in Stochastic Reaction-Diffusion-Dynamics Model.
A) A regular kinetochore (KT) representation. Blue bead represents the main body of the KT and orange wall section is the outer KT, which is covered by grid of Ndc80 seeds. B) Schematic of the KT model. Grid of beads within corona are connected to each other inside some cut-off radius (200 nm); all of them are also attached to the center of mass of the corresponding KT. This set of features was made to keep the KT shape constant. C) In the Stochastic Reaction-Diffusion-Dynamics model, we can vary the curvature of the outer KT from the most curved (beads cover the KT surface) to almost flat. D) Another option of the model is to cover a KT with molecular “armor” blocking the MT access to KTs, thus mimicking the role of CH arms. E) A more detailed look at the KT-MT interface. Here MTs are lime tubes. MTs are attached to the corona with the help of Ndc80-complexes (cyan lines). F) Types of KT-MT attachments are illustrated by examples coming from snapshots taken from the simulations.