The catalytic mechanism of the mitochondrial methylenetetrahydrofolate dehydrogenase/cyclohydrolase (MTHFD2)
Fig 7
(a) Superimposition of the NAD(P)+ binding pockets with regards to the dehydrogenase activity. The C atoms of MTHFD1 (PDB: 6ECQ) are colored in brown, and those of MTHFD2 (PDB: 6KG2) are colored in white. Switching from Ser197 to Arg233 defines a key force in binding 2′-phosphate of NADP+ rather than phosphate ion (Pi). (b) Superimposition of the MTHFD2 and MTHFD2L NAD+ binding pockets. Key residues involved in binding are shown as sticks and colored red for MTHFD2 and pink for MTHFD2L.