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Interpreting and de-noising genetically engineered barcodes in a DNA virus

Fig 7

Barcode length distributions for 90%, 99%, and 99.9% sequencing reads cutoffs.

Applying a cutoff for barcode abundance eliminates most recall of longer barcodes that were not intended in the original design for a 12-nucleotide barcode library. These barcodes are derived from the L3 clustering. The y-axis is square root-transformed so low values are more visible.

Fig 7

doi: https://doi.org/10.1371/journal.pcbi.1010131.g007