Nanoscale organization of ryanodine receptor distribution and phosphorylation pattern determines the dynamics of calcium sparks
Fig 8
Spark properties for disrupted geometries G4 and G5.
For each setup 200 single simulations were conducted. Green: inner; red: uniform; blue: outer; gray: no phosphorylation. Light colours: 20% phosphorylation; dark colours: 50% phosphorylation. (A) Activation map with the first opening time of each RyR for a representative simulation for uniform phosphorylation, G4 (left) and G5 (right). ‘x’ indicates that the RyR did not open during the simulation. red circle: initial open RyR. (B) Spark fidelity for none, inner, uniform, and outer phosphorylation in G4. The black bars indicate the 95% Agresti-Coull confidence interval. (C) Spark fidelity for none, inner, uniform, and outer phosphorylation in G5. The black bars indicate the 95% Agresti-Coull confidence interval. (D) Amplitude (mean ± standard error) for none, inner, uniform, and outer phosphorylation in G4 (no phosphorylation n = 3, inner 20% n = 37, inner 50% n = 77, uniform 20% n = 15, uniform 50% n = 60, outer 20% n = 5, outer 50% n = 24). (E) TTP (mean ± standard error) for none, inner, uniform, and outer phosphorylation in G4. (F) Spark duration (mean ± standard error) for none, inner, uniform, and outer phosphorylation in G4. *=p<0.05, **=p<0.01 and ***=p<0.001. The p values were calculated with respect to the non phosphorylated case using a t-test, except for the fidelity p value which was calculated from Fisher’s exact test (due to binary data).