Nanoscale organization of ryanodine receptor distribution and phosphorylation pattern determines the dynamics of calcium sparks
Fig 6
Effect of phosphorylation pattern on Ca2+ spark properties in G1.
For each pattern, 200 simulations were conducted. green: inner, red: uniform, blue: outer. Lighter shades: 20% phosphorylation, darker shades: 50% phosphorylation. (A) Spark fidelity for inner, uniform, and outer phosphorylation patterns in G1. The black bars indicate the 95% Agresti-Coull confidence interval. (B) Scatter plot comparing spark duration and amplitude. Circles: single simulations, crosses: mean values. (C) Spark amplitude (mean ± standard error) for inner, uniform, and outer phosphorylation patterns in G1. (For no phosphorylation n = 63, for inner 20% n = 97, for inner 50% n = 125, for uniform 20% n = 94, for uniform 50% n = 121, for outer 20% n = 63, and for outer 50% n = 118). (D) TTP (mean ± standard error) for inner, uniform, and outer phosphorylation patterns in G1. (E) Spark duration (mean ± standard error) for inner, uniform, and outer phosphorylation patterns in G1. (F) Intensity timecourse for successful sparks with inner phosphorylation. (G) Intensity timecourse for successful sparks with uniform phosphorylation. (H) Intensity timecourse for successful sparks with outer phosphorylation. (I) Mean open time for each RyR throughout 200 simulations—inner 50% phosphorylation. (J) Mean open time for each RyR throughout 200 simulations—uniform 50% phosphorylation. (K) Mean open time for each RyR throughout 200 simulations—outer 50% phosphorylation. **=p<0.01 and ***=p<0.001. The p values were calculated with respect to the non phosphorylated case using a t-test, except for the fidelity p value which was calculated from Fisher’s exact test (due to binary data).