Nanoscale organization of ryanodine receptor distribution and phosphorylation pattern determines the dynamics of calcium sparks
Fig 4
Spark properties of unphosphorylated RyR in geometries G1 and G2.
For each geometry 200 simulations were conducted. (A) Intensity over time for G1 calcium sparks. Dark gray: successful sparks (n = 63), light dotted gray lines: failed sparks. (B) Intensity over time for G2 calcium sparks. Gray: successful sparks (n = 29), light dotted gray lines: failed sparks. (C) The probability of spark generation (spark fidelity) for G1 and G2. Error bars indicate the 95% Agresti-Coull confidence interval. (D) Spark amplitude (mean ± standard error) for successful sparks in G1 and G2. (E) Average time to peak (TTP) (mean ± standard error) for G1 and G2, in ms. (F) Scatter plot of spark amplitude versus TTP. The crosses represent the mean values across all simulations for G1 and G2. (G) Histogram tracking total number of opened RyRs across individual simulations for G1 and G2. For example, for geometry G1 all 50 RyRs are opened in 18 simulations, whereas for G2 no simulations had 50 open RyRs. ***=p<0.001. The p values were calculated from a t-test, except for the fidelity p value which was calculated from Fisher’s exact test (due to binary data).