LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress
Fig 6
Simulating the BY4742 cell cycle with active spindle checkpoint arrest (Misaligned_Spindle and Unattached_Kinetochores nodes fixed at ’2’) to assess the role of lnc10883 overexpression (’Over’) in silico under these conditions (the fourth experimental model simulation in Fig 1).
The box color indicates the node levels in each simulation state. The simulation presented a cyclic attractor related to a functional cell cycle, which includes all states depicted on the X-axis. Thus, the functional cell cycle is evidenced when the simulation outcomes cyclic attractors presenting the activation of all phenomenological nodes, further inhibited when the MITOSIS_EXIT node reaches ’2’, and restarting the cell cycle (MASS returning to ’0’), as observed here (see details in the ’Model cycling rationale’ in the Materials and Methods). The upper picture in the box on the left is the arrest mechanism mediated by spindle checkpoints, while the bottom picture reports our suggested arrest skip mechanism. The red ’X’ on the edges depicts the suppression of given regulations.