Pairing statistics and melting of random DNA oligomers: Finding your partner in superdiverse environments
Fig 2
Double strand vs. random sequence DNA melting.
Ensemble melting curve as a function of temperature. Green dots: measured ensemble melting of 12N at crsDNA = 0.04 g/l. Shading marks experimental uncertainty resulting from the average over 8 experimental replicas. Dashed lines: theoretical melting predicted for equimolar solutions of two complementary 12mers at cDNA = 0.02 g/l each (dashed blue line) and cDNA = 0.04/412 ≈ 2.4 10−9 g/l each (dashed red line). 12N θe(T) exhibits a behavior intermediate between the two. Dashed lines are obtained by averaging many melting curves of complementary 12mers. cNaCl = 1M in all curves.