In vitro machine learning-based CAR T immunological synapse quality measurements correlate with patient clinical outcomes
Fig 1
Comparable CAR expressions between patient #3 and patient #4.
PBMCs from patients #3 and #4 were transduced with the kappa-CAR retrovirus, respectively. The ratio and expression (MFI) of CD8 and CD4 subsets are calculated. (A) Flow cytometry analysis of CD8 and CD4 positive population from patients #3 and #4. (B) The ratio of CD3 and CAR positive subsets is calculated. (C) Different subsets of CD3+ T cells and viability are summarized. Data are pooled from at least two independent experiments.