RNA-Seq is not required to determine stable reference genes for qPCR normalization
Fig 6
Differential expression of target genes between P3 and P21 mouse sciatic nerves assessed by qPCRs and RNA-Seq.
The target genes (Mki67, p75NTR, Sox2, Mbp, Mpz, Cd90) have been normalized with the normalization factor (NF) computed from conventional reference genes Hsp60 & Ppia in the first column (A, D, G, J, M, P) or from RNA-Seq derived reference genes Ppp3ca and Coq9 in the second column (B, E, H, K, N, Q). The third column contains the Fold Changes computed from RNA-Seq (C,F,I,L,O,R). The normalized counts for each gene in each group were used to calculate the fold changes in RNA-Seq. The WT group was used as the experimental control. The Padj value of RNA-Seq is indicated in the P21 group for each gene in the RNA-Seq column. For the qPCRs, Non-parametric Mann Whitney U test was used to assess differences between the groups. The alpha value was set at 0.05 and P values are annotated as follows: * P<0.05, ** P<0.01, *** P<0.001.