RNA-Seq is not required to determine stable reference genes for qPCR normalization
Fig 4
Non-normalized expression profiles (2-ΔCq) of reference genes derived from RNA-Seq analysis of sciatic nerves at post-natal day 3 (P3) and 21 (P21).
P3 group is the experimental calibrator. (A) Fkbp4, (B) Lama2, (C) Leprotl1, (D) Supt4a, (E) Coq9, (F) Chmp2a, (G) Ank2, (H) Vcp, (I) Laptm5, (J) Ppp3ca, and (K) Normalisation Factor (Ppp3ca + Coq9). Non-parametric Mann Whitney U test was used to assess differences between the groups. The alpha value was set at 0.05 and P values are annotated as follows: * P<0.05, ** P<0.01, *** P<0.001.