RNA-Seq is not required to determine stable reference genes for qPCR normalization
Fig 2
Non-normalized expression profiles (2-ΔCq) of conventional human reference genes in iPSC derived microglia.
WT group is used as the experimental calibrator. (A) ACTB, (B) GAPDH, (C) GUSB, (D) PPIA, (E) RPLP0, (F) TBP, (G) HPRT, (H) PGK1, (I) UBC, (J) RPL13A, and (K) Normalization Factor (NF) assessed by NormFinder that combines RPLP0 & GUSB. Non-parametric Mann Whitney U test was used to assess differences between the groups. The alpha value was set at 0.05 and P values are annotated as follows: * P<0.05, ** P<0.01, *** P<0.001.