Regulatory network-based imputation of dropouts in single-cell RNA sequencing data
Fig 5
Detection of cell type-specific transcription factors is improved upon network-based imputation.
A) Enrichment score in GO term “DNA-binding transcription factor activity” among the genes uniquely detected after each imputation approach. B) Projection of cells onto a low dimension representation of the data before imputation, using ZINB-WaVE[30]. Color represents normalized expression levels of EHF (top) and OSR1 (bottom) before and after Network-based imputation. DEC: definitive endoderm cells; EC: endothelial cells; H9: undifferentiated human embryonic stem cells; HFF: human foreskin fibroblasts; NPC: neural progenitor cells; TB: trophoblast-like cells.