Bias-free estimation of information content in temporally sparse neuronal activity
Fig 6
Validating the bias-correction methods on real data by tracking the same neurons across multiple sessions.
(A) Naïve SI as a function of the sampling duration for a 2-hour-long concatenated session, for real (blue) and shuffled (black) data. Inset, the same for simulated data, with the true SI shown in cyan. (B) Estimated SI as a function of sample duration from a 20-minute-long subsample for the naïve calculation (blue), shuffle (black), SR (red), SSR (magenta), and the full 2-hour-long concatenated session SI (cyan). (C) Estimated SI from a 20-minute-long subsample for the naïve calculation (blue), AE (orange), BAE (green), and the full 2-hour-long concatenated session (cyan). Data in A-C show the mean across the recorded cells from one example mouse. (D) Bias-corrected SI for individual neurons using SR (red) and SSR (magenta) on 20-minute subsamples versus the naïve SI of the full concatenated 2-hour-long session. (E) Bias-corrected SI for individual neurons using AE (orange) and BAE (green) on 20-minute subsamples versus the naïve SI of the full concatenated 2-hour-long session. (F) Estimated SI of the same neurons for the BAE method versus the SSR method. Inset, discrepancy between the estimated SI using SSR versus BAE. Data in D-F are for 947 place cells that were pooled from N = 9 mice (25–254 cells per mouse), tracked across 6 imaging sessions in the same environment and found to be active in at least 5 of those sessions.