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Analytical kinetic model of native tandem promoters in E. coli

Fig 8

Promoter occupancy ω estimated for the step model.

(A) ω as a function of the rate constant for a free RNAP to bind to the unoccupied promoter (k_bind⋅[R]) and of the time for that RNAP to start elongation after commitment to OC, . The horizontal black line at ω = 1, is the maximum fraction of time that the promoter can be occupied (i.e., the maximum promoter occupancy). (B) k_occlusion plotted as a function of ω and d_TSS. Since k_occlusion increases with ω if and only if d_TSS ≤ 35, it renders the simultaneous occupation of both TSS’s impossible.

doi: https://doi.org/10.1371/journal.pcbi.1009824.g008