Analytical kinetic model of native tandem promoters in E. coli
Fig 6
Empirical data and analytical model of how dTSS influences the single-cell protein numbers of genes controlled by tandem promoters.
(A) Mean, (B) CV2, and (C) S of single protein numbers in the 1X media as a function of dTSS. (D), (E), and (F) show the same for the 0.5X media, respectively. Each red dot is the mean from 3 biological repeats for a pair of promoters (S2 Table). The dots were also grouped in 3 ‘boxes’ based on their dTSS. In each box, the red line is the median and the top and bottom are the 3rd and 1st quartiles, respectively. The vertical black bars are the range between minimum and maximum of the red dots. In A, all lines are best fits. In B, C, D, E, and F, all lines are model predictions, based on the parameters used to best fit A. The insets show the R2 for each model fit and prediction.