Analytical kinetic model of native tandem promoters in E. coli
Fig 4
Single cell protein numbers by microscopy and flow-cytometry.
(A) Example single-cell distributions (3 biological replicates) of fluorescence (in arbitrary units) of cells with a YFP tagged gene controlled by a pair of tandem promoters obtained by flow-cytometry, ‘FC’. (B) Example confocal microscopy image of cells overlapped by the results of cell segmentation from the corresponding phase contrast image. The two white arrows show the dimensions of the image, for scaling purposes. (C) Mean single-cell protein fluorescence of 10 genes (Table G in the S3 Appendix) when obtained by FC plotted against when obtained by microscopy, ‘Mic’. (D) Mean single-cell protein fluorescence (own measurements) plotted against the corresponding mean single-cell protein numbers reported in [28]. From the equation of the best fitting line without y-intercept (y-intercept = 0), we obtained a scaling factor, sf, equal to 0.09.