Analytical kinetic model of native tandem promoters in E. coli
Fig 2
(I) We identified genes controlled by tandem promoters in Regulon DB. (II) Next, we measured the single-cell protein levels of those genes with arrangements I and II that are tagged in the YFP strain library [28]. We also measured the mean RNA fold changes of these genes over time (S1 Appendix, section ‘RNA-seq measurements and data analysis’). (III) We used the single-cell data to tune the model. (IV) Finally, we used the model to explore the state space of protein expression. Figure created with BioRender.com.