Analytical kinetic model of native tandem promoters in E. coli
Fig 1
Interference between tandem promoters with different arrangements relative to each other.
(A) Interference by an RNAP occupying the downstream promoter on the activity of the elongating RNAP from upstream promoter. The TSSs need to be at least 36 bp apart (the length occupied by an RNAP when in OC, [23,25]) (B) Interference by occlusion of one of the promoter’s TSS by an RNAP on the TSS of the other promoter. The distance between the TSSs need to be ≤ 35 bp apart. Blue clouds are RNAPs. Black arrows sit on TSSs and point towards the direction of transcription elongation. Arrangements (I-II) of two promoters studied in the manuscript in tandem formation are represented. The red rectangles are the protein coding regions. We studied only the natural tandem promoters that neither overlap with nor have in between another gene (arrangements I and II, which differ based on whether the promoter regions overlap or not). Other arrangements (not considered in this study) are shown in Fig A in the S2 Appendix. Figure created with BioRender.com.