Extracting multi-way chromatin contacts from Hi-C data
Fig 5
Comparing triplet contact predictions with SPRITE.
(A) Genes, RNA-seq and H3K27ac ChIP-seq signals [60, 67] in a 0.45 Mb region on human chr18 with the positions of three types of SOIs (Enhancers, Promoters and Super-enhancers). (B) log10(pij) from Hi-C compared with that from HLM at 5-kb resolution. (C) The triplet contact frequency from SPRITE is compared with log10(pijk) from HLM anchored at an active promoter site. (D) SPRITE frequency of triplets which are divided into four quantiles based on ascending order of pijk. (E) For three sites, i < j < k, along a polymer chain, the minor and major sections denoted by m and t, respectively, are depicted with dashed lines. (F) The expected three-body contact probability , and (G) the specificity Z-score
. (H) The mean specificity Z-score of triplet contacts with respect to all possible combinations of annotations (E: Enhancers, P: Promoters, S: Super-enhancers, #: sites without any annotation). The error bars are the standard deviations.