Fine-grained, nonlinear registration of live cell movies reveals spatiotemporal organization of diffuse molecular processes
Fig 7
Cell symmetry breaking reveals polarization dependent organization of Profilin coherence a) Time course of a drug-induced symmetry breaking experiment, following the protocols published in [39]. 500 s prior to imaging cells seeded on glass slides are treated with 25 μM Blebbistatin. During the initial phase after drug induction the cells exhibit a stable morphology (not filmed). The remainder of the displacement time course (total cell edge displacement integrated over the cell boundary) displays distinct phases of cell morphodynamic activity, as indicated. Symmetry breaking occurs 150–250 s into the movie. b) Remapped mNG-Actin signals at four select time points. See Fig 1b for the original image sequence. The cell registration relied on Halo-CAAX as a location fiducial. c) Actin entropy computed over a rolling window of 25 time points. d) Profilin time-series coherence score computed over a rolling window of 25 time points. Both the Actin entropy and Profilin coherence reveal a shift in high values away from the cell front to the cell center and back during the symmetry breaking process showing that Profilin organization is responsive to changes in subcellular Actin polymerization. See text for further discussion. All scale bars 10 μm.