Fine-grained, nonlinear registration of live cell movies reveals spatiotemporal organization of diffuse molecular processes
Fig 5
Profilin dynamics in live cells show patterns in local time-series coherence.
Raw intensity vs local time-series coherence scores of diverse Profilin probes. The computation of reliable coherence scores is enabled by the remapping of the movie into a spatially stationary reference frame. a) Control experiment using an mCherry cytoplasmic volume marker. As expected, coherence of a diffuse signal is high across the entire cell footprint. For remapping, a Halo-CAAX tag membrane marker was used as the location fiducial. b) Cell undergoing drug-induced symmetry breaking, which expresses SNAP-Profilin at endogenous levels. For remapping, a Halo-CAAX tag membrane marker was used as the location fiducial. c) WT EGFP-Profilin (top) and R88E mApple-Profilin mutant (bottom) concurrently expressed at low concentration from a leaky CMV100 promotor in a Profilin knockout cell. For remapping, the lowpass-filtered Actin-SNAP signal was used as the location fiducial. WT and R88E Profilin display distinct coherence patterns. d) Quantification of subcellular heterogeneity of the signals observed in a-c using the Gini coefficient. Dots indicate values for individual cells. All scale bars 10 μm.