Multifunctional RNA-binding proteins influence mRNA abundance and translational efficiency of distinct sets of target genes
Fig 3
Multifunctional RBPs regulate translation of distinct sets of target genes.
(A) Heatmap with Glass’ △ scores quantifying the effect size of the witness effects for mRNA and TE correlations. Both effect sizes are significant for a highlighted set of 21 multifunctional RBPs. For this set of RBPs, individual Venn Diagrams representing the overlap in the total number of mRNA and TE targets are displayed. (B) Bar plot quantifying the magnitude of mRNA and TE effect size (Glass’ △ scores) for multifunctional RBPs. RBP effect sizes are largely independent of the mode of regulation. (C) Selected histograms and dot plots illustrating the significance of RBP-target correlations and the enrichment of GO terms for the targets bound by 4 multifunctional RBPs: DDX3X, G3BP1, PUM1, and UCHL5. For each RBP, the 12 most significant parental GO terms are displayed. For three of the RBPs, mRNA and TE targets exhibit different enrichment of significant GO terms. (D) Box plots with transcript, 5’ UTR, CDS, and 3’ UTR sequence lengths in nucleotides for mRNA and TE targets corresponding to the four selected multifunctional RBPs in (C). A total of 9 multifunctional RBPs bind targets with significantly different CDS lengths (Wilcoxon rank sum test). See also S3 Fig.