Unsupervised discovery of dynamic cell phenotypic states from transmitted light movies
Fig 6
Calculation of morphological state transition probabilities by cell linkage analysis.
(A) Cell pairs found in proximity across on successive time points were linked (left). Cell linkages, along with assigned morphological states of linked cells, were used to calculate transition probabilities between all states. (B) Heatmap shows transition probability matrix between all morphological clusters (left); image montage shows representative cell tracks identified from the culture (right). Scale bar represents 10 μm. (C) Distribution of cellular velocity for linked cells for each morphological cluster. (D) Plot shows mean cell velocity against mean cell eccentricity for each morphological cluster.