A conserved expression signature predicts growth rate and reveals cell & lineage-specific differences
Fig 7
Growth-rate and cell-type specific effects of mitochondria inhibitors on proliferation rate, cell viability and cell state.
(A) Schematic of the experimental setup for measuring the effects of mitochondria inhibitors on slow and fast proliferating cells. (B) Fast proliferating Fibroblast and ESCs sorted by CFSE signal maintained a higher fraction of cells in S phase over two days of growth in medium+DMSO. (C) Effect of antimycin treatment on fast and slow proliferating fibroblasts and ESCs (Drug effect: log2 of the fraction of cells in S-phase when treated with DMSO divided by the fraction of cells in S-phase when treated with drug). (D) Fast fibroblasts changed morphology after the treatment with oligomycin. Scale bars = 80 μm. (E) Immunostaining of fibroblasts for N-Cadherin and DAPI after drug treatment and corresponding quantifications. Fast fibroblasts lose N-Cadherin staining specifically after oligomycin treatment. Scale bars = 15 μm. The fluorescence intensity of N-Cadherin has been quantified on the right. Medians and the 95% confidence intervals are shown as error bars. Kruskal–Wallis test was used for statistical comparison (ns, not significant, **** p < 0.0001). (F) Effect of oligomycin treatment on fibroblast viability. The % viable cells is measured as % trypan blue-negative cells.