A conserved expression signature predicts growth rate and reveals cell & lineage-specific differences
Fig 6
Expression of proliferation-related gene sets in cells sorted by intra-population heterogeneity in mitochondria membrane potential.
(A) Cells were stained with Hoechst and CFSE and a homogenous population of equally sized cells in G1 with equal CFSE was obtained by FACS. These cells were stained with TMRE sorted by TMRE, and then used for RNA-seq, or allowed to proliferate to measure the doubling time of each TMRE sub-population. (B, C) Enrichment maps of fibroblasts and ESCs sorted by TMRE. (D) Doublings times, as estimated by the measured by the decrease in CFSE signal over time, for high, medium and low TMRE sorted cells. P-values are from ANOVA, testing if TMRE is predictive of doubling time (see Materials and Methods).