A particle-based computational model to analyse remodelling of the red blood cell cytoskeleton during malaria infections
Fig 7
Comparison of experimental and simulated pair cross-correlations.
(A) STED images of an exposed RBC membrane at the trophozoite stage (28–36 hours post malaria infection). Red and green fluorescence signals correspond to KAHRP and ankyrin sites on the RBC membrane. (B) Pair cross-correlation (PCC) between KAHRP and ankyrin computed using the two-color images obtained at different hours post malaria infection. (C) PCC between KAHRP and N-terminus of β-spectrin. Images and PCC values in (A,B) are taken from [35]. (D) KAHRP (red) and ankyrin (green) fluorescence signal is constructed from location points obtained from simulations (overlaid white points). (E) PCC between KAHRP and ankyrin is computed from two-color images generated from simulations for different binding energy between KAHRP and actin as indicated by the color bar. (F) Same as (E) for the KAHRP and N-terminus of spectrin PCC. (G) The map shows PCC values at zero distance for KAHRP-actin pairs (top panel) and KAHRP-ankyrin pairs (bottom panel) at different binding rates between KAHRP and ankyrin/actin sites for average actin filament length 24 nm. The white dots mark the cases where a peak is observed at a finite distance. (H) and (I) Same as (G) for average actin filament length 36 nm and 48 nm respectively.